Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells.
Methods
; 59(1): 71-9, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23079396
ABSTRACT
The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells using microfluidic arrays. As part of a study exploring the effect of genotype on Wnt pathway activation, data were collected for 96 qPCR assays on 1440 lymphoblastoid cells. The description of methods includes preliminary data processing steps. The methods used in the collection and analysis of single-cell qPCR data are contrasted with those used in conventional qPCR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Células Progenitoras Linfoides
/
Análise de Célula Única
/
Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article