Ultrabright photoactivatable fluorophores created by reductive caging.
Nat Methods
; 9(12): 1181-4, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23103881
ABSTRACT
Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)-10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbocianinas
/
Corantes Fluorescentes
/
Microscopia de Fluorescência
Limite:
Humans
Idioma:
En
Ano de publicação:
2012
Tipo de documento:
Article