Biolistics-based gene silencing in plants using a modified particle inflow gun.
Methods Mol Biol
; 940: 63-74, 2013.
Article
em En
| MEDLINE
| ID: mdl-23104334
ABSTRACT
RNA interference (RNAi) is one of the most commonly used techniques for examining the function of genes of interest. In this chapter we present two examples of RNAi that use the particle inflow gun for delivery of the DNA constructs. In one example transient RNAi is used to show the function of an anthocyanin regulatory gene in flower petals. In the second example stably transformed cell cultures are produced with an RNAi construct that results in a change in the anthocyanin hydroxylation pattern.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Solanum tuberosum
/
Biolística
/
Antirrhinum
/
Interferência de RNA
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article