Characterization of the alkaloids in goldenseal (Hydrastis canadensis) root by high resolution Orbitrap LC-MS(n).
Anal Bioanal Chem
; 405(13): 4487-98, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23180081
ABSTRACT
Liquid chromatography coupled to multistage mass spectrometry (LC-MS(n)) is being used increasingly in pharmaceutical research and for quality control in herbal medicines because of its superior sensitivity and selectivity. In this study, a rapid, high-resolution liquid chromatography-mass spectrometry (LC-MS(n)) method was developed to separate and identify alkaloids in the root extract of goldenseal, which is one of the 20 most popular herbal supplements used worldwide. In total, 28 alkaloids were separated and characterized including one novel compound and 21 identified, or tentatively identified, for the first time in goldenseal. The current high-resolution LC-MS(n) method provides a rapid and definitive means of profiling the composition of goldenseal root and will provide a useful tool in understanding the bioactivity of this medicinal plant.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
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Extratos Vegetais
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Raízes de Plantas
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Hydrastis
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Alcaloides
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article