Dynamics of free versus complexed ß2-microglobulin and the evolution of interfaces in MHC class I molecules.
Immunogenetics
; 65(3): 157-72, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23229474
ABSTRACT
In major histocompatibility complex (MHC) class I molecules, monomorphic ß(2)-microglobulin (ß(2)m) is non-covalently bound to a heavy chain (HC) exhibiting a variable degree of polymorphism. ß(2)M can stabilize a wide variety of complexes ranging from classical peptide binding to nonclassical lipid presenting MHC class I molecules as well as to MHC class I-like molecules that do not bind small ligands. Here we aim to assess the dynamics of individual regions in free as well as complexed ß(2)m and to understand the evolution of the interfaces between ß(2)m and different HC. Using human ß(2)m and the HLA-B*2709 complex as a model system, a comparison of free and HC-bound ß(2)m by nuclear magnetic resonance spectroscopy was initially carried out. Although some regions retain their flexibility also after complex formation, these studies reveal that most parts of ß(2)m gain rigidity upon binding to the HC. Sequence analyses demonstrate that some of the residues exhibiting flexibility participate in evolutionarily conserved ß(2)m-HC contacts which are detectable in diverse vertebrate species or characterize a particular group of MHC class I complexes such as peptide- or lipid-binding molecules. Therefore, the spectroscopic experiments and the interface analyses demonstrate that ß(2)m fulfills its role of interacting with diverse MHC class I HC as well as effector cell receptors not only by engaging in conserved intermolecular contacts but also by falling back upon key interface residues that exhibit a high degree of flexibility.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectroscopia de Ressonância Magnética
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Antígeno HLA-B27
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Microglobulina beta-2
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article