Quantitative optical trapping on single organelles in cell extract.
Nat Methods
; 10(1): 68-70, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23241632
ABSTRACT
We have developed an optical trapping method to precisely measure the force generated by motor proteins on single organelles of unknown size in cell extract. This approach, termed VMatch, permits the functional interrogation of native motor complexes. We apply VMatch to measure the force, number and activity of kinesin-1 on motile lipid droplets isolated from the liver of normally fed and food-deprived rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Organelas
/
Cinesinas
/
Metabolismo dos Lipídeos
/
Pinças Ópticas
/
Lipídeos
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article