ifet-1 is a broad-scale translational repressor required for normal P granule formation in C. elegans.
J Cell Sci
; 126(Pt 3): 850-9, 2013 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-23264733
ABSTRACT
Large cytoplasmic ribonucleoprotein germ granule complexes are a common feature in germ cells. In C. elegans these are called P granules and for much of the life-cycle they associate with nuclear pore complexes in germ cells. P granules are rich in proteins that function in diverse RNA pathways. Here we report that the C. elegans homolog of the eIF4E-transporter IFET-1 is required for oogenesis but not spermatogenesis. We show that IFET-1 is required for translational repression of several maternal mRNAs in the distal gonad and functions in conjunction with the broad-scale translational regulators CGH-1, CAR-1 and PATR-1 to regulate germ cell sex determination. Furthermore we have found that IFET-1 localizes to P granules throughout the gonad and in the germ cell lineage in the embryo. Interestingly, IFET-1 is required for the normal ultrastructure of P granules and for the localization of CGH-1 and CAR-1 to P granules. Our findings suggest that IFET-1 is a key translational regulator and is required for normal P granule formation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Caenorhabditis elegans
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Poro Nuclear
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Grânulos Citoplasmáticos
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Proteínas de Caenorhabditis elegans
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Células Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article