Enrichment of methylated DNA by methyl-CpG immunoprecipitation.
Methods Mol Biol
; 971: 201-12, 2013.
Article
em En
| MEDLINE
| ID: mdl-23296965
ABSTRACT
Normal DNA methylation is an epigenetic modification required for proper development. Aberrant DNA methylation, in contrast, is frequently observed in many different malignancies including leukemias and lymphomas. Global DNA methylation profiling addresses the methylated sequences (methylome) of patient genomes to identify disease-specific methylation patterns. Workload in methylome analyses can be considerably reduced by methylome enrichment using proteins or antibodies with high affinity to methylated DNA. Methyl-CpG Immunoprecipitation (MCIp) employs an immobilized recombinant human methyl-CpG binding domain protein 2, MBD2, which binds methylated CpGs in double-stranded DNA. Elution with increasing salt concentrations allows the fractionated enrichment of different degrees of methylation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ilhas de CpG
/
Metilação de DNA
/
Imunoprecipitação
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article