The outer membrane of Gram-negative bacteria: lipid A isolation and characterization.
Methods Mol Biol
; 966: 239-258, 2013.
Article
em En
| MEDLINE
| ID: mdl-23299739
ABSTRACT
The isolation and characterization of the lipid A domain of lipopolysaccharide (LPS) are important methodologies utilized to gain understanding of the Gram-negative cell envelope. Here, we describe protocols often employed by our laboratory for small- and large-scale isolation of lipid A from bacterial cells. Additionally, we describe various methodologies including isolation of radiolabeled lipid A, thin layer chromatography, and various mass spectrometry methods. Tandem mass spectrometry is an integral tool for the structural characterization of lipid A molecules, and both coventional collision induced dissociation (CID) and new ultraviolet photodissociation (UVPD) methods are described.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
Lipídeo A
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article