Postmortem biopsy to obtain lung tissue in congenital diaphragmatic hernia.
Neonatology
; 103(3): 213-7, 2013.
Article
em En
| MEDLINE
| ID: mdl-23328121
ABSTRACT
BACKGROUND:
The accrual of human tissues from autopsies for diagnostic and translational research has decreased significantly over the last decades.OBJECTIVES:
The objective of this study was to evaluate our experience with lung biopsy through a minithoracotomy as an alternative for obtaining postmortem tissue when full autopsy is refused in congenital diaphragmatic hernia (CDH) patients.METHODS:
Within 2 h of death we routinely asked parents for permission to perform an autopsy. Starting in 2001, parents who refused autopsy were asked permission for a postmortem lung biopsy. Pathology autopsy and biopsy reports were compared to clinical records.RESULTS:
Between 2001 and 2009, 46 patients died from CDH. Permission for autopsy was granted in 5 patients (11%). Of the remaining 41 patients, the parents of 15 (33%) agreed to postmortem lung biopsy. In all cases, additional findings were reported from the autopsy or biopsy, without changing the originally reported cause of death. In 1 case, we isolated fibroblasts from the lung biopsy using standardized cell culture techniques. Parents were able to take their child home with a minimal delay following biopsy.CONCLUSIONS:
Parents refusing a full autopsy frequently agree to postmortem organ biopsy. This approach should therefore be considered as a valuable alternative, when permission for full autopsy is declined, for obtaining human tissues for both diagnostic and research purposes and is potentially applicable to other anomalies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hérnias Diafragmáticas Congênitas
/
Pulmão
Limite:
Female
/
Humans
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Male
/
Newborn
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article