Turn-on fluorescence detection of apoptotic cells using a zinc(II)-dipicolylamine-functionalized poly(diacetylene) liposome.
Chem Asian J
; 8(4): 755-9, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-23341205
ABSTRACT
A liposome-based fluorescence sensing system for apoptotic cells has been developed from stimuli-responsive poly(diacetylene)-liposomes for the first time. The combination of the liposome components, a phosphatidylserine-binding Zn(II)-dipicolylamine component and an alcohol-terminated component in the ratio of 21, has led to an efficient detection system for apoptotic cells, as demonstrated by confocal fluorescence microscopy and FACS analysis. The liposome shows a color change from blue to reddish purple and emits fluorescence in the turn-on mode upon interaction with phosphatidylserine. The present system thus avoids the washing steps required for "always-on"-type sensing systems.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Picolínicos
/
Polímeros
/
Zinco
/
Apoptose
/
Poli-Inos
/
Aminas
/
Lipossomos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article