Synergistic and tunable human gene activation by combinations of synthetic transcription factors.
Nat Methods
; 10(3): 239-42, 2013 Mar.
Article
em En
| MEDLINE
| ID: mdl-23377379
ABSTRACT
Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Calicreínas
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Antígeno Carcinoembrionário
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Engenharia Genética
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Ativação Transcricional
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Antígeno Prostático Específico
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Receptor ErbB-2
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article