A novel EPS-producing strain of Bacillus licheniformis isolated from a shallow vent off Panarea Island (Italy).
Curr Microbiol
; 67(1): 21-9, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23397221
ABSTRACT
A haloalkaliphilic, thermophilic Bacillus strain (T14), isolated from a shallow hydrothermal vent of Panarea Island (Italy), produced a new exopolysaccharide (EPS). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T14 was highly related (99 % similarity) to Bacillus licheniformis DSM 13(T) and Bacillus sonorensis DSM 13779(T). Further DNA-DNA hybridization analysis revealed 79.40 % similarity with B. licheniformis DSM 13(T) and 39.12 % with B. sonorensis DSM 13779(T). Sucrose (5 %) was the most efficient carbon source for growth and EPS production. The highest EPS production (366 mg l(-1)) was yielded in fermenter culture at 300 rpm after 48 h of incubation. The purified fraction EPS1 contained fructose/fucose/glucose/galactosamine/mannose in a relative proportion of 1.00.750.28trtr and possessed a molecular weight of 1,000 kDa displaying a trisaccharide unit constituted by sugars with a ß-manno-pyranosidic configuration. Screening for biological activity showed anti-cytotoxic effect of EPS1 against Avarol in brine shrimp test, indicating a potential use in the development of novel drugs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Bacillus
/
Fontes Hidrotermais
Limite:
Animals
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article