New insights from existing sequence data: generating breakthroughs without a pipette.
Mol Cell
; 49(4): 605-17, 2013 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-23438857
ABSTRACT
With the rapidly declining cost of data generation and the accumulation of massive data sets, molecular biology is entering an era in which incisive analysis of existing data will play an increasingly prominent role in the discovery of new biological phenomena and the elucidation of molecular mechanisms. Here, we discuss resources of publicly available sequencing data most useful for interrogating the mechanisms of gene expression. Existing next-generation sequence data sets, however, come with significant challenges in the form of technical and bioinformatic artifacts, which we discuss in detail. We also recount several breakthroughs made largely through the analysis of existing data, primarily in the RNA field.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise de Sequência de DNA
/
Mineração de Dados
/
Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article