DNA shuffling of cytochrome P450 enzymes.
Methods Mol Biol
; 987: 177-88, 2013.
Article
em En
| MEDLINE
| ID: mdl-23475678
ABSTRACT
DNA family shuffling is an efficient method for creating libraries of novel enzymes, in which a high proportion of mutants exhibit correct folding and possess catalytic properties distinct from the starting material. The evolutionary arrangement of cytochromes P450 into subfamilies of enzymes with highly similar nucleotide sequences but distinct catalytic properties renders them excellent starting material for DNA family shuffling experiments. This chapter provides a general method for creating libraries of shuffled P450s from two or more related sequences and incorporates several recent improvements to previously published methods.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema Enzimático do Citocromo P-450
/
Embaralhamento de DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article