Differentiation of serum-free embryoid bodies from human induced pluripotent stem cells into networks.
Stem Cell Res
; 10(3): 454-63, 2013 May.
Article
em En
| MEDLINE
| ID: mdl-23500645
ABSTRACT
Three-dimensional aggregation cultures allow for complex development of differentiated human induced pluripotent stem cells. However, this approach is not easily amenable to live-cell imaging and electrophysiological applications due to the thickness and the geometry of the tissue. Here, we present an improvement on the traditional aggregation method by combining the use of cell culture inserts with serum-free embryoid bodies (SFEBs). The use of this technique allows the structures to maintain their three-dimensional structure while thinning substantially. We demonstrate that this technique can be used for electrophysiological recodings as well as live-cell calcium imaging combined with electrical stimulation, akin to organotypic slice preparations. This provides an important experimental tool that can be used to bridge 3-D structures with traditional monolayer approaches used in stem cell applications.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes Induzidas
/
Corpos Embrioides
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article