Enzyme-mediated nutrient release: glucose-precursor activation by ß-galactosidase to induce bacterial growth.
Org Biomol Chem
; 11(17): 2903-10, 2013 May 07.
Article
em En
| MEDLINE
| ID: mdl-23519143
ABSTRACT
Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. To demonstrate this principle, we developed a simple model system, which mimics how bacteria exploit natural carbon sources. A masked glucose precursor that is activated by ß-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium. No bacterial growth was observed in the presence of control substances in which ß-galactosidase mediated cleavage did not lead to glucose release. This study represents a proof-of-principle example in which a bacterium can grow in a nutrient-free medium by inducible, enzyme-mediated nutrient release from a precursor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Beta-Galactosidase
/
Escherichia coli
/
Glucose
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article