An efficient and mild access to N-acetyl protected purine nucleosides based on a chemoselective enzymatic hydrolysis.
J Biotechnol
; 165(2): 99-101, 2013 May 20.
Article
em En
| MEDLINE
| ID: mdl-23524256
ABSTRACT
N-Monoacetylated derivatives of ribo- (adenosine, guanosine) and 2'-deoxyribonucleosides (2'-deoxyadenosine and 2'-deoxyguanosine), useful as oligonucleotide building blocks, were obtained in 88-100% by enzymatic chemoselective hydrolysis of the corresponding peracetylated nucleosides. Among the tested hydrolases, most satisfactory results were found with acylase I from Aspergillus melleus and Candida antarctica lipase B. For acylase I, the observed chemoselectivity towards ester hydrolysis, without amide reaction, broadens the information about the selectivity of the enzyme and its synthetic applications in the field of nucleosides.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nucleosídeos de Purina
/
Aspergillus
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article