Detoxification of malachite green by Pleurotus florida laccase produced under solid-state fermentation using agricultural residues.
Environ Technol
; 34(1-4): 139-47, 2013.
Article
em En
| MEDLINE
| ID: mdl-23530324
ABSTRACT
Laccase was produced from Pleurotus florida under solid-state fermentation, and the production was optimized by response surface methodology. The predicted maximum laccase production of 8.81 U g(-1) was obtained by the optimum concentration of malt extract, banana peel, wheat bran and CuSO4, which was found to be 0.69 g, 10.61 g, 10.68 g and 77.15 ppm, respectively. The validation results suggested that the laccase production was 7.96 U g(-1) in the optimized medium, which was close to the predicted value. Decolorization efficiency of P. florida laccase was evaluated against malachite green (MG). Rapid decolorization of MG dye was observed, and a dark-coloured precipitate was formed in the reaction mixture. HPLC analysis indicated that the laccase enzyme degraded MG by the demethylation process. The toxicity of MG was reduced to 67% after the treatment with laccase, which was confirmed by a phytotoxicity study.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corantes de Rosanilina
/
Produtos Agrícolas
/
Pleurotus
/
Lacase
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article