Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies.
ACS Appl Mater Interfaces
; 5(8): 3323-8, 2013 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-23537161
ABSTRACT
This Article presents the development of a combined cell culture-biosensing platform using vertically aligned self-assembled peptide nanofibers. Peptide nanofibers were patterned on a microchip containing gold microelectrodes to provide the cells with a 3D environment enabling them to grow and proliferate. Gold microelectrodes were functionalized with conductive polymers for the electrochemical detection of dopamine released from PC12 cells. The combined cell culture-biosensing platform assured a close proximity of the release site, the cells and the active surface of the sensor, thereby rendering it possible to avoid a loss of sensitivity because of the diffusion of the sample. The obtained results showed that the peptide nanofibers were suitable as a cell culturing substrate for PC12 cells. The peptide nanofibers could be employed as an alternative biological material to increase the adherence properties of PC12 cells. Dopamine was amperometrically detected at a value of 168 fmole.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Técnicas Biossensoriais
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Técnicas de Cultura de Células
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Nanofibras
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article