Paraffin sections of 70-100 µm: a novel technique and its benefits for studying the nervous system.
J Neurosci Methods
; 215(2): 241-4, 2013 May 15.
Article
em En
| MEDLINE
| ID: mdl-23537935
ABSTRACT
This technical communication provides a protocol for processing paraffin-embedded tissue blocks from the nervous system sectioned at 70-100 µm. Staining is performed on free-floating sections. Unlike 4-15 µm thick paraffin sections, this technique permits (1) the accurate recognition of specific cell types and cytoarchitectonic units (subcortical nuclei and cortical layers) and, owing to the superimposition of large numbers of biological structures, (2) the accurate assessment of pathological changes, e.g., those associated with synucleinopathies, tauopathies, and TDP-43 proteinopathies.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inclusão em Parafina
/
Sistema Nervoso
Limite:
Adult
/
Aged
/
Aged80
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article