Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer.
Methods Mol Biol
; 1006: 181-96, 2013.
Article
em En
| MEDLINE
| ID: mdl-23546792
ABSTRACT
The ABI PRISM (®) 377 DNA Sequencer is used for a variety of microsatellite-based research. The platform provides researchers with a cost-effective means for high-throughput genotyping, which can be further optimized by multiplexing microsatellite loci or by using a tail-labeling approach to screen large sets of markers. The goals of this chapter are to present a protocol for performing microsatellite-based analyses on the ABI 377 and to provide researchers with information on how to troubleshoot common issues associated with running the ABI 377 sequencers.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
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Análise de Sequência de DNA
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Repetições de Microssatélites
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Biologia Molecular
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article