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Real-time evaluation of binding mechanisms in multivalent interactions: a surface plasmon resonance kinetic approach.
Munoz, Eva Maria; Correa, Juan; Riguera, Ricardo; Fernandez-Megia, Eduardo.
Afiliação
  • Munoz EM; Department of Organic Chemistry and Center for Research in Biological Chemistry and Molecular Materials, University of Santiago de Compostela, Jenaro de la Fuente s/n, 15782 Santiago de Compostela, Spain. evamaria.munoz@usc.es
J Am Chem Soc ; 135(16): 5966-9, 2013 Apr 24.
Article em En | MEDLINE | ID: mdl-23565759
Multivalency is a key, ubiquitous phenomenon in nature characterized by a complex combination of binding mechanisms, with special relevance in carbohydrate-lectin recognition. Herein we introduce an original surface plasmon resonance kinetic approach to analyze multivalent interactions that has been validated with dendrimers as monodisperse multivalent analytes binding to lectin clusters. The method, based on the analysis of early association and late dissociation phases of the sensorgrams provides robust information of the glycoconjugate binding efficiency and real-time structural data of the binding events under the complex scenario of the glyco-cluster effect. Notably, it reveals the dynamic nature of the interaction and offers experimental evidence on the contribution of binding mechanisms.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ressonância de Plasmônio de Superfície Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ressonância de Plasmônio de Superfície Idioma: En Ano de publicação: 2013 Tipo de documento: Article