Probing the sulfhydryl groups of nuclear matrix proteins with 6-iodoacetamidofluorescein.
Cell Biol Int Rep
; 14(5): 409-18, 1990 May.
Article
em En
| MEDLINE
| ID: mdl-2357731
ABSTRACT
Rat liver nuclear matrices were reacted with the fluorescent dye 6-iodoacetamidofluorescein and the matrix proteins were then separated by one and two-dimensional polyacrylamide gel electrophoresis. Upon transillumination with U.V. light it was possible to see that several proteins had reacted with the dy, thus indicating the presence of free -SH groups. This labelling technique allowed the detection of a large number of proteins, being several folds more sensitive than conventional Coomassie Blue staining, as demonstrated by two-dimensional electrophoretical separation. If nuclear matrices were treated with reducing agents before being reacted with 6-iodoacetamidofluorescein, the fluorescence increased with about the same intensity in all the protein bands. It is proposed that 6-iodoacetamidofluorescein can be used as a specific and very sensitive probe to study the -SH groups of nuclear matrix proteins.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos de Sulfidrila
/
Proteínas Nucleares
/
Matriz Nuclear
/
Fluoresceínas
Limite:
Animals
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article