Self-assembled DNA nanopores that span lipid bilayers.
Nano Lett
; 13(6): 2351-6, 2013 Jun 12.
Article
em En
| MEDLINE
| ID: mdl-23611515
ABSTRACT
DNA nanotechnology excels at rationally designing bottom-up structures that can functionally replicate naturally occurring proteins. Here we describe the design and generation of a stable DNA-based nanopore that structurally mimics the amphiphilic nature of protein pores and inserts into bilayers to support a steady transmembrane flow of ions. The pore carries an outer hydrophobic belt comprised of small chemical alkyl groups which mask the negatively charged oligonucleotide backbone. This modification overcomes the otherwise inherent energetic mismatch to the hydrophobic environment of the membrane. By merging the fields of nanopores and DNA nanotechnology, we expect that the small membrane-spanning DNA pore will help open up the design of entirely new molecular devices for a broad range of applications including sensing, electric circuits, catalysis, and research into nanofluidics and controlled transmembrane transport.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Nanoestruturas
/
Bicamadas Lipídicas
Tipo de estudo:
Clinical_trials
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article