Enterococcal Rgg-like regulator ElrR activates expression of the elrA operon.
J Bacteriol
; 195(13): 3073-83, 2013 Jul.
Article
em En
| MEDLINE
| ID: mdl-23645602
ABSTRACT
The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óperon
/
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
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Enterococcus faecalis
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article