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Expression of aquaporins in human embryos and potential role of AQP3 and AQP7 in preimplantation mouse embryo development.
Xiong, Yun; Tan, Ya-Jing; Xiong, Yi-Meng; Huang, Yi-Ting; Hu, Xiao-Ling; Lu, Yong-Chao; Ye, Ying-Hui; Wang, Ting-Ting; Zhang, Dan; Jin, Fan; Huang, He-Feng; Sheng, Jian-Zhong.
Afiliação
  • Xiong Y; Key Laboratory of Reproductive Genetics, Zhejiang University, Ministry of Education, Hangzhou, China.
Cell Physiol Biochem ; 31(4-5): 649-58, 2013.
Article em En | MEDLINE | ID: mdl-23652731
BACKGROUND/AIMS: Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis. METHODS: RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2∼8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their mRNA expression and protein expression in the oocyte, zygote, 2-cell embryo, 4-cell embryo, 8-cell embryo, morula, and blastocyst from both human and mouse using RT-PCR and immunofluorescence, respectively. RESULTS: AQP3 and AQP7 were detected in all these stages. Knockdown of either AQP3 or AQP7 by targeted siRNA injection into 2-cell mouse embryos significantly inhibited preimplantation embryo development. However, knockdown of AQP3 in JAr spheroid did not affect its attachment to Ishikawa cells. CONCLUSION: These data demonstrate that multiple aquaporins are expressed in the early stage human embryos and that AQP3 and AQP7 may play a role in preimplantation mouse embryo development.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aquaporinas / Embrião de Mamíferos / Aquaporina 3 Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aquaporinas / Embrião de Mamíferos / Aquaporina 3 Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article