A label-free amplified fluorescence DNA detection based on isothermal circular strand-displacement polymerization reaction and graphene oxide.

Li, Zhen; Zhu, Wenping; Zhang, Jinwen; Jiang, Jianhui; Shen, Guoli; Yu, Ruqin

Li, Zhen; Zhu, Wenping; Zhang, Jinwen; Jiang, Jianhui; Shen, Guoli; Yu, Ruqin.

Afiliação

Li Z; State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Analyst ; 138(13): 3616-20, 2013 Jul 07.

Article em En | MEDLINE | ID: mdl-23671905

ABSTRACT

ABSTRACT

A label-free fluorescent DNA biosensor has been presented based on isothermal circular strand-displacement polymerization reaction (ICSDPR) combined with graphene oxide (GO) binding. The proposed method is simple and cost-effective with a low detection limit of 4 pM, which compares favorably with other GO-based homogenous DNA detection methods.

Assuntos

Técnicas Biossensoriais/métodos; DNA/análise; DNA/química; Grafite/química; Óxidos/química; Polimerização; Temperatura; Técnicas Biossensoriais/economia; Análise Custo-Benefício; Limite de Detecção; Espectrometria de Fluorescência

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxidos / Temperatura / DNA / Técnicas Biossensoriais / Polimerização / Grafite Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2013 Tipo de documento: Article

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