The effects of bupivacaine injection and oral nitric oxide on extraocular muscle in the rabbit.
Graefes Arch Clin Exp Ophthalmol
; 251(9): 2227-33, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23733036
ABSTRACT
AIM:
Nitric oxide (NO) plays a key role in muscle regeneration, which is the primary response, observed during bupivacaine-induced extraocular muscle (EOM) hypertrophy. Our aims were to investigate the effects of bupivacaine injection into the rabbit EOM and the interaction with NO. MATERIALS ANDMETHODS:
Superior rectus (SR) muscles of 24 New Zealand albino rabbits were studied. Single muscle twitch tension (SMTT) and tetanic muscle tensions at 50, 75, and 100 Hz were recorded using a 15 V stimuli. The rabbits were equally allocated into three groups. Measurements were performed without any drug treatments in group 1. In groups 2 and 3, bupivacaine, 0.5 ml of a 0.50 % solution, was injected into the EOM, and after 21 days, measurements were performed. Oral isosorbide dinitrate (NO donor) at 20 mg/day was given each day prior to measurements in group 3.RESULTS:
SMTTs were 69.9 (66.7-77.6), 187.7 (114.9-252.1) and 204.2 (135.3-311.6) mg in groups 1, 2, and 3 respectively. SMTTs for both groups 2 and 3 were significantly higher than that for group 1 (p < 0.05). Compared with group 1, group 2 exhibited a 3.8-11.7 % increase in the tetanic tensions at 50, 75, and 100 Hz, but none of these differences were statistically significant. The increase was 47.5-137.5 % in group 3 relative to group 2, and the differences were statistically significant except at 100 Hz. The enlargement of the muscle fibers after bupivacaine injection was shown histopathologically.CONCLUSION:
Bupivacaine injection increased the EOM tension in rabbits to some extent. NO augmented the effect of bupivacaine.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bupivacaína
/
Doadores de Óxido Nítrico
/
Dinitrato de Isossorbida
/
Anestésicos Locais
/
Músculos Oculomotores
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article