MT1-MMP prevents growth inhibition by three dimensional fibronectin matrix.
Biochem Biophys Res Commun
; 436(3): 503-8, 2013 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-23756810
ABSTRACT
The extracellular microenvironment plays a key role in regulation of cellular functions and growth control. We show here that membrane-type 1 matrix metalloproteinase (MT1-MMP) acts as a growth promoter in confluent culture. When MT1-MMP was silenced in HT1080 fibrosarcoma cells, cells created three dimensional (3D) fibronectin matrix in a confluent culture, and growth of cells embedded within it was retarded. Formation of 3D fibronectin matrix initiated by MT1-MMP silencing was impeded by knockdown of either FN or integrin ß1, which resulted in restoration of cell growth. When cells in 3D fibronectin matrix were treated with integrin ß1 inhibitory antibody, cells underwent S phase entry. These results suggest that MT1-MMP prevents growth suppression by 3D fibronectin matrix, which is mediated through integrin ß1.
Palavras-chave
3D; DMEM; Dulbecco's modified Eagle's medium; ECM; ERK; FN; Growth; Integrin; MAPK; MMP; MT1-MMP; PBS; Tumor; extracellular matrix; extracellular signal-regulated kinase; fibronectin; matrix metalloproteinase; membrane-type 1 MMP; mitogen-activated protein kinase; pRb; phosphate-buffered saline; retinoblastoma protein; siRNA; small interfering RNA; three-dimensional
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibronectinas
/
Integrina beta1
/
Matriz Extracelular
/
Metaloproteinase 14 da Matriz
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article