Interleukin-1ß suppresses activity of an inwardly rectifying K+ channel in human renal proximal tubule cells.
J Physiol Sci
; 63(5): 377-87, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23797607
ABSTRACT
We investigated the effect of interleukin-1ß (IL-1ß) on activity of an inwardly rectifying K+ channel in cultured human proximal tubule cells (RPTECs), using the patch-clamp technique and Fura-2 Ca2+ imaging. IL-1ß (15 pg/ml) acutely reduced K+ channel activity in cell-attached patches. This effect was blocked by the IL-1 receptor antagonist (20 ng/ml), an inhibitor of phospholipase C, neomycin (300 µM), and an inhibitor of protein kinase C (PKC), GF109203X (500 nM). The Fura-2 Ca2+ imaging revealed that IL-1ß increased intracellular Ca2+ concentration even after removal of extracellular Ca2+, which was blocked by an inhibitor of inositol 1,4,5-trisphosphate receptors, 2-aminoethoxydiphenyl borate (2-APB, 1 µM). Moreover, IL-1ß suppressed channel activity in the presence of 2-APB without extracellular Ca2+. These results suggest that IL-1ß suppresses K+ channel activity in RPTECs through binding to its specific receptor and activation of the PKC pathway even though intracellular Ca2+ does not increase.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais de Potássio Corretores do Fluxo de Internalização
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Interleucina-1beta
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Túbulos Renais Proximais
Limite:
Adult
/
Humans
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Male
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article