The two-component system CBO2306/CBO2307 is important for cold adaptation of Clostridium botulinum ATCC 3502.
Int J Food Microbiol
; 167(1): 87-91, 2013 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-23810493
ABSTRACT
Clostridium botulinum is a notorious foodborne pathogen. Its ability to adapt to and grow at low temperatures is of interest for food safety. Two-component systems (TCSs) have been reported to be involved in cold-shock and growth at low temperatures. Here we show the importance of TCS CBO2306/CBO2307 in the cold-shock response of C. botulinum ATCC 3502. The relative expression levels of the cbo2306 and cbo2307 were up to 4.4-fold induced in the cold-shocked cultures but negatively regulated in the late-log and stationary growth phase in relation to early logarithmic growth phase in non-shocked cultures. Importance of the CBO2306/CBO2307 in the cold stress was further demonstrated by impaired growth of insertional cbo2306 or cbo2307 knockout mutants in relation to the wild-type strain ATCC 3502. The results suggest that the TCS CBO2306/CBO2307 is important for cold-shock response and adaptation of C. botulinum ATCC 3502 to low temperature.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adaptação Fisiológica
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Clostridium botulinum
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Temperatura Baixa
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Genes Bacterianos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article