The role of transforming growth factor ß1 in fractional laser resurfacing with a carbon dioxide laser.

ABSTRACT

The aim of this study was to investigate the role of transforming growth factor ß1 in mechanisms of cutaneous remodeling induced by fractional carbon dioxide laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO2 laser treatment. Biopsies were taken at 1 h and at 1, 3, 7, 14, 21, 28, and 56 days after treatment. Transforming growth factor (TGF) ß1 expression in skin samples was evaluated by ELISA, dermal thickness by hematoxylin-eosin staining, collagen and elastic fibers by Ponceau S and Victoria blue double staining, and types I and III collagens by ELISA. The level of TGF ß1 in the laser-treated areas of skin was significantly increased compared with that in the control areas on days 1 (p < 0.05), 3 (p < 0.01), and 7 (p < 0.05) and then decreased by day 14 after treatment, at which time it had returned to the baseline level. Dermal thickness and the amount of type I collagen of the skin of the laser-treated areas had increased significantly (p < 0.05) compared with that in control areas on days 28 and 56. Fibroblast proliferation showed a positive correlation with TGF ß1 expression during the early stages (r = 0.789, p < 0.01), and there was a negative correlation between the level of TGF ß1 and type I collagen in the late stages, after laser treatment (r = -0.546, p < 0.05). TGF ß1 appears to be an important factor in fractional laser resurfacing.