Fluorescent dye cocktail for multiplex drug-site mapping on human serum albumin.
ACS Comb Sci
; 15(9): 452-7, 2013 Sep 09.
Article
em En
| MEDLINE
| ID: mdl-23941241
ABSTRACT
Elucidating how molecules bind to HSA is fundamental for predicting drug incompatibilities. Through combinatorial screening, we identified a novel fluorescent dye (BD140) with turn-on fluorescence emission and specific binding at HSA drug site 2. We further combined it with dansylamide to develop a fluorescent dye cocktail for high-throughput mapping of the interaction between therapeutics at HSA drug-binding sites.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Albumina Sérica
/
Preparações Farmacêuticas
/
Ensaios de Triagem em Larga Escala
/
Corantes Fluorescentes
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article