Gene synthesis method based on overlap extension PCR and DNAWorks program.
Methods Mol Biol
; 1073: 9-17, 2013.
Article
em En
| MEDLINE
| ID: mdl-23996435
ABSTRACT
Gene synthesis by chemical methods provides a powerful tool for modifying genes and exploring their structure, expression, and function in the post-genomic era. However, a bottleneck in recent gene synthesis technologies is the high cost of oligonucleotide synthesis and post-synthesis sequencing. Here, we describe a simple, rapid, and low-cost gene synthesis method based on overlap extension PCR (OE-PCR) and the DNAWorks program. This method enables DNA sequences with sizes ranging from 200 bp to 3 kb to be synthesized with few errors, and these errors can be easily corrected by site-directed mutagenesis. Thus, it is amenable to automation for the multiplexed synthesis of different genes and has a potential for high-throughput gene synthesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Software
/
DNA
/
Reação em Cadeia da Polimerase
/
Biologia Computacional
/
Genes Sintéticos
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article