Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ).
Plant J
; 76(4): 718-27, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-23998490
ABSTRACT
Next-generation whole-genome shotgun assemblies of complex genomes are highly useful, but fail to link nearby sequence contigs with each other or provide a linear order of contigs along individual chromosomes. Here, we introduce a strategy based on sequencing progeny of a segregating population that allows de novo production of a genetically anchored linear assembly of the gene space of an organism. We demonstrate the power of the approach by reconstructing the chromosomal organization of the gene space of barley, a large, complex and highly repetitive 5.1 Gb genome. We evaluate the robustness of the new assembly by comparison to a recently released physical and genetic framework of the barley genome, and to various genetically ordered sequence-based genotypic datasets. The method is independent of the need for any prior sequence resources, and will enable rapid and cost-efficient establishment of powerful genomic information for many species.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hordeum
/
Análise de Sequência de DNA
/
Genoma de Planta
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Mapeamento de Sequências Contíguas
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Genômica
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article