Plasticicumulans lactativorans sp. nov., a polyhydroxybutyrate-accumulating gammaproteobacterium from a sequencing-batch bioreactor fed with lactate.
Int J Syst Evol Microbiol
; 64(Pt 1): 33-38, 2014 Jan.
Article
em En
| MEDLINE
| ID: mdl-24002473
ABSTRACT
A bacterial consortium that accumulated more than 90â% (w/w) polyhydroxybutyrate (PHB) from lactate was selected in a laboratory-scale bioreactor with a 'feast-famine' regime. Bacterial strain YD(T), representing a dominant species in this enrichment, was isolated and characterized. Analysis of the 16S rRNA gene sequence revealed that the isolate is a member of the class Gammaproteobacteria, forming an independent phylogenetic lineage. The closest relative of the isolate was Plasticicumulans acidivorans TUD-YJ37(T), with 94â% 16S rRNA gene sequence similarity. Strain YD(T) was an obligate aerobe with large, ovoid, Gram-negative cells, motile by means of a polar flagellum. It utilized a relatively broad spectrum of substrates (e.g. carbohydrates, fatty acids) as carbon and energy sources. The temperature range for growth was 20-45 °C, with an optimum at 40 °C; the pH range was pH 6.0-8.0, with an optimum at pH 7.0. The major respiratory lipoquinones were Q-8 (91â%) and Q-7 (9â%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and an unidentified aminolipid. The predominant fatty acids in the membrane polar lipids were C16ââ1ω7c, C16ââ0 and C18ââ1ω7c. The G+C content of the genomic DNA was 68.5 mol%. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolate is proposed to represent a novel species in the genus Plasticicumulans, for which the name Plasticicumulans lactativorans sp. nov. is proposed. The type strain is YD(T) (â=âDSM 25287(T)â=âNCCB 100398(T)).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Poliésteres
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Ácido Láctico
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Reatores Biológicos
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Gammaproteobacteria
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Hidroxibutiratos
País/Região como assunto:
Europa
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article