[HPLC and UPLC-MS detection of 5-HMF from rabbit ncurolymph after treated with Cornus officinalis].

ABSTRACT

OBJECTIVE: To detect 5-HMF from rabbit ncurolymph after given different dosage of Cornus officinalis via intragastric administration by HPLC and UPLC-MS. METHODS: Rabbit ncurolymph was cramped out three days after given low, medium, and high dosage of Cornus officinalis. 5-HMF from rabbit ncurolymph was detected with HPLC and UPLC-MS, respectively. RESULTS: 5-HMF from rabbit ncurolymph was detected with HPLC method only in rabbits given high dose group. Meanwhile, 5-HMF could be detected with UPLC-MS method in rabbits given medium as well as low dose group. CONCLUSION: These results suggest that 5-HMF can cross the blood brain barrier (BBB) of rabbits and enter the rabbit ncurolymph.