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High clustering rates of multidrug-resistant Mycobacterium tuberculosis genotypes in Panama.
Rosas, Samantha; Bravo, Jaime; Gonzalez, Franklin; de Moreno, Nora; Sanchez, Joel; Gavilan, Ronnie G; Goodridge, Amador.
Afiliação
  • Rosas S; Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP), Ciudad Del Saber, Panama, Panama. agoodridge@indicasat.org.pa.
BMC Infect Dis ; 13: 442, 2013 Sep 23.
Article em En | MEDLINE | ID: mdl-24053690
ABSTRACT

BACKGROUND:

Tuberculosis continues to be one of the leading causes of death worldwide and in the American region. Although multidrug-resistant tuberculosis (MDR-TB) remains a threat to TB control in Panama, few studies have focused in typing MDR-TB strains. The aim of our study was to characterize MDR Mycobacterium tuberculosis clinical isolates using PCR-based genetic markers.

METHODS:

From 2002 to 2004, a total of 231 Mycobacterium tuberculosis isolates from TB cases country-wide were screened for antibiotic resistance, and MDR-TB isolates were further genotyped by double repetitive element PCR (DRE-PCR), (GTG)5-PCR and spoligotyping.

RESULTS:

A total of 37 isolates (0.85%) were resistant to both isoniazid (INH) and rifampicin (RIF). Among these 37 isolates, only two (5.4%) were resistant to all five drugs tested. Dual genotyping using DRE-PCR and (GTG)5-PCR of MDR Mycobacterium tuberculosis isolates revealed eight clusters comprising 82.9% of the MDR-TB strain collection, and six isolates (17.1%) showed unique fingerprints. The spoligotyping of MDR-TB clinical isolates identified 68% as members of the 42 (LAM9) family genotype.

CONCLUSION:

Our findings suggest that MDR Mycobacterium tuberculosis is highly clustered in Panama's metropolitan area corresponding to Panama City and Colon City, and our study reveals the genotype distribution across the country.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Resistente a Múltiplos Medicamentos / Farmacorresistência Bacteriana Múltipla / Mycobacterium tuberculosis / Antituberculosos Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: America central / Panama Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose Resistente a Múltiplos Medicamentos / Farmacorresistência Bacteriana Múltipla / Mycobacterium tuberculosis / Antituberculosos Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: America central / Panama Idioma: En Ano de publicação: 2013 Tipo de documento: Article