Three complementary techniques for the clarification of temperature effect on low-density lipoprotein-chondroitin-6-sulfate interaction.
Anal Biochem
; 443(2): 139-47, 2013 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-24055620
ABSTRACT
A rigorous processing of adsorption data from quartz crystal microbalance technology was successfully combined with the data obtained by partial filling affinity capillary electrophoresis and molecular dynamics for the clarification of the temperature effect on the interaction of a major glycosaminoglycan chain chondroitin-6-sulfate (C6S) of proteoglycans with low-density lipoprotein (LDL) and with a peptide fragment of apolipoprotein B-100 (residues 3359-3377 of LDL, PPBS). Two experimental techniques and computational atomistic methods demonstrated a nonlinear pattern of the affinity of C6S at temperatures above 38.0 °C to both LDL and PPBS. The temperature affects the interaction of C6S with LDL and PPBS by influencing the structural behavior of glycosaminoglycan C6S and/or that of LDL.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfatos de Condroitina
/
Lipoproteínas LDL
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article