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Comparison of heat inactivation and cell disruption protocols for identification of mycobacteria from solid culture media by use of vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Machen, Alexandra; Kobayashi, Miwako; Connelly, Mary Robin; Wang, Yun F Wayne.
Afiliação
  • Machen A; Emory University School of Medicine.
J Clin Microbiol ; 51(12): 4226-9, 2013 Dec.
Article em En | MEDLINE | ID: mdl-24068013
Two novel protocols for inactivation and extraction were developed and used to identify 107 Mycobacterium clinical isolates, including Mycobacterium tuberculosis complex, from solid cultures using Vitek matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The protocol using heat inactivation with sonication and cell disruption with glass beads resulted in 82.2% and 88.8% species and genus level identifications, respectively.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Manejo de Espécimes / Tuberculose / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Mycobacterium Tipo de estudo: Diagnostic_studies / Evaluation_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Manejo de Espécimes / Tuberculose / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Mycobacterium Tipo de estudo: Diagnostic_studies / Evaluation_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2013 Tipo de documento: Article