Bond elasticity controls molecular recognition specificity in antibody-antigen binding.
Nano Lett
; 13(11): 5197-202, 2013 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-24074342
ABSTRACT
Force-spectroscopy experiments make it possible to characterize single ligand-receptor pairs. Here we measure the spectrum of bond strengths and flexibilities in antibody-antigen interactions using optical tweezers. We characterize the mechanical evolution of polyclonal antibodies generated under infection and the ability of a monoclonal antibody to cross-react against different antigens. Our results suggest that bond flexibility plays a major role in remodeling antibody-antigen bonds in order to improve recognition during the maturation of the humoral immune system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reações Antígeno-Anticorpo
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article