Microscale chaotic advection enables robust convective DNA replication.
Anal Chem
; 85(21): 10536-41, 2013 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-24083802
ABSTRACT
The ability of chaotic advection under microscale confinement to direct chemical processes along accelerated kinetic pathways has been recognized for some time. However, practical applications have been slow to emerge because optimal results are often counterintuitively achieved in flows that appear to possess undesirably high disorder. Here we present a 3D time-resolved analysis of polymerase chain reaction (PCR)-mediated DNA replication across a broad ensemble of geometric states. The resulting parametric map reveals an unexpectedly wide operating regime where reaction rates remain constant over 2 orders of magnitude of the Rayleigh number, encompassing virtually any realistic PCR condition (temperature, volume, gravitational alignment), a level of robustness previously thought unattainable in the convective format.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dinâmica não Linear
/
Replicação do DNA
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article