Nano-anchors with single protein capacity produced with STED lithography.
Nano Lett
; 13(11): 5672-8, 2013.
Article
em En
| MEDLINE
| ID: mdl-24111646
ABSTRACT
Acrylate nanoanchors of subdiffraction-limited diameter are written with optical stimulated emission depletion (STED) lithography. After incubation, 98% of all nanoanchors are loaded quickly with fluorescently labeled antibodies. Controlling the size of the nanoanchors allows for limiting the number of the antibodies. Direct stochastic optical reconstruction microscopy (dSTORM) imaging, statistical distribution of fluorescence, quantitative fluorescence readout, and single molecule blinking consistently prove that 80% of the nanoanchors with a 65 nm diameter are carrying only one antibody each, which are functional as confirmed with live erythrocytes.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acrilatos
/
Nanotecnologia
/
Nanoestruturas
/
Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article