Detergent-free mass spectrometry of membrane protein complexes.
Nat Methods
; 10(12): 1206-8, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-24122040
ABSTRACT
We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Detergentes
/
Lipídeos
/
Proteínas de Membrana
/
Micelas
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article