Simultaneous and confirmative detection of multi-residues of ß2-agonists and ß-blockers in urine using LC-MS/MS/MS coupled with ß-receptor molecular imprinted polymer SPE clean-up.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
; 30(12): 2093-101, 2013.
Article
em En
| MEDLINE
| ID: mdl-24138591
ABSTRACT
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using ß-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 ß2-agonists and 21 ß-blockers in urine samples. Urine samples were subjected to enzymatic hydrolysis by ß-glucoronidase/arylsulphatase, and then extracted with perchloric acid. Sample clean-up was performed using ß-receptor MIP SPE. A Supelco Ascentis® express Rp-Amide column was used to separate the analytes, and MS³ detection used an electrospray ionisation source in positive-ion mode. Recovery studies were carried out using blank urine samples fortified with the 46 analytes at the levels of 0.5, 1.0 and 2.0 µg l⻹. Recoveries were obtained ranging from 60.1% to 109.9% with relative standard deviations (RSDs, n = 7) from 0.5% to 19.4%. The limits of detection (LODs) and limits of quantitation (LOQs) of the 46 analytes in urine were 0.02-0.18 and 0.05-0.60 µg l⻹, respectively. As a result of the selective clean-up by MIP SPE and MS³ detection of the target drugs, the sensitivity and accuracy of the present method was high enough for monitoring ß2-agonist and ß-blocker residues in urine samples. Satisfactory results were obtained in the process of the determination of positive urine samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Detecção do Abuso de Substâncias
/
Receptores Adrenérgicos beta
/
Agonistas Adrenérgicos beta
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Antagonistas Adrenérgicos beta
/
Proteínas Imobilizadas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article