Building a pseudo-atomic model of the anaphase-promoting complex.
Acta Crystallogr D Biol Crystallogr
; 69(Pt 11): 2236-43, 2013 Nov.
Article
em En
| MEDLINE
| ID: mdl-24189235
ABSTRACT
The anaphase-promoting complex (APC/C) is a large E3 ubiquitin ligase that regulates progression through specific stages of the cell cycle by coordinating the ubiquitin-dependent degradation of cell-cycle regulatory proteins. Depending on the species, the active form of the APC/C consists of 14-15 different proteins that assemble into a 20-subunit complex with a mass of approximately 1.3 MDa. A hybrid approach of single-particle electron microscopy and protein crystallography of individual APC/C subunits has been applied to generate pseudo-atomic models of various functional states of the complex. Three approaches for assigning regions of the EM-derived APC/C density map to specific APC/C subunits are described. This information was used to dock atomic models of APC/C subunits, determined either by protein crystallography or homology modelling, to specific regions of the APC/C EM map, allowing the generation of a pseudo-atomic model corresponding to 80% of the entire complex.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Modelos Moleculares
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Proteínas de Saccharomyces cerevisiae
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Ciclossomo-Complexo Promotor de Anáfase
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Subunidade Apc1 do Ciclossomo-Complexo Promotor de Anáfase
Limite:
Humans
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article