Secure splenic delivery of plasmid DNA and its application to DNA vaccine.
Biol Pharm Bull
; 36(11): 1800-6, 2013.
Article
em En
| MEDLINE
| ID: mdl-24189423
ABSTRACT
In this experiment, we developed a novel safe and effective gene delivery vector coated with γ-polyglutamic acid (γ-PGA-coated complexes). The γ-PGA-coated complex was composed of chiseled spherical nano-particles with anionic charges. The plasmid DNA/polyethyleneimine complex (non-coated complex) showed high transgene efficiency in the spleen and lung after intravenous administration in mice, with high liver toxicity and lethality. On the other hand, γ-PGA-coated complex selectively showed high transgene efficiency in the spleen without such toxicity. Furthermore, the γ-PGA-coated complex highly accumulated and showed high gene expression in the marginal zone of the spleen. Those results strongly indicated that γ-PGA-coated complex was suitable as a DNA vaccine vector. We therefore applied γ-PGA-coated complex to melanoma DNA vaccine, pUb-M. The γ-PGA-coated complex containing pUb-M significantly inhibited the growth and metastasis of a melanoma cell line, B16-F10 cells. In conclusion, we developed a splenic gene vector, γ-PGA-coated complex, as a novel technology for clinical vaccination.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Poliglutâmico
/
Baço
/
Técnicas de Transferência de Genes
/
Vacinas Anticâncer
/
Vacinas de DNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2013
Tipo de documento:
Article