Effect of anionic macromolecules on intestinal permeability of furosemide.
Drug Dev Ind Pharm
; 41(2): 190-3, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-24191750
ABSTRACT
CONTEXT Furosemide is an anionic molecule and has very low absorption in gastro intestinal tract. OBJECTIVE:
The aim of this study was to investigate the effect of anionic macromolecules on the intestinal permeability of Furosemide. MATERIALS ANDMETHODS:
The intestinal permeability of Furosemide was determined using single-pass intestinal perfusion technique in rats. Briefly a jejunal segment of â¼10 cm was isolated and cannulated in both ends for inlet and outlet solution. The perfusate was collected every 10 min and samples were analyzed using the RP-HPLC method. Test samples containing furosemide and two anionic macromolecules, sodium carboxy methyl cellulose and sodium alginate, at different concentrations were used.RESULTS:
The obtained data showed that existence of Sodium carboxy methyl cellulose significantly increased the Peff values in all three investigated concentrations (p < 0.05) but sodium alginate only in concentrations <0.1% increased drug permeability.DISCUSSION:
It is concluded that the anionic macromolecules at specific concentrations could alter the permeability of anionic drugs across the biological membranes.CONCLUSIONS:
Donnan phenomenon and chelating property of macromolecules could be attributed to the observed effect.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Furosemida
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article