Processes that affect electrospray ionization-mass spectrometry of nucleobases and nucleosides.
J Am Soc Mass Spectrom
; 7(11): 1106-8, 1996 Nov.
Article
em En
| MEDLINE
| ID: mdl-24203072
ABSTRACT
Due to the complexity of electrospray ionization processes and the many factors that affect the ion signal, optimization of electrospray ionization methods to gain ultimate sensitivity for analysis of nucleobases and nucleosides may not be straightforward. In this work, we investigated the effect of the pK a and the gas-phase basicity of analyte and other electrolytes on the [M+H](+) ion signal for 11 select nucleobases and nucleosides in 50% methanolwater solution. Solution chemistry plays a role in the electrospray signal for all analytes, but gas-phase chemistry may be important for compounds with pK a <3 depending on the solution composition. For compounds with pK a <3, gas-phase proton transfer reactions can be promoted to increase analyte electrospray response by the addition of ammonium acetate to the solution.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1996
Tipo de documento:
Article